| 초록 |
Zingiber officinale Roscoe has been widely used as a folk medicine to treat various diseases, including cancer. This study aims to re-examine the therapeutic potential of co-administration of natural products and cancer chemotherapeutics. Candidate material for this project, ${ alpha}$ -zingiberene, was extracted from Zingiber officinale Roscoe, and ${ alpha}$ -zingiberene makes up $35.02{ pm}0.30%$ of its total essential oil. ${ alpha}$ -Zingiberene showed low $IC_{50}$ values, $60.6{ pm}3.6$ , $46.2{ pm}0.6$ , $172.0{ pm}6.6$ , $80.3{ pm}6.6$ ( ${ mu}g/mL$ ) in HeLa, SiHa, MCF-7 and HL-60 cells each. These values are a little bit higher than $IC_{50} $ values of general essential oil in those cells. The treatment of ${ alpha}$ -zingiberene produced nucleosomal DNA fragmentation in SiHa cells, and the percentage of sub-diploid cells increased in a concentration-dependent manner in SiHa cells, hallmark features of apoptosis. Mitochondrial cytochrome c activation and an in vitro caspase-3 activity assay demonstrated that the activation of caspases accompanies the apoptotic effect of ${ alpha}$ -zingiberene, which mediates cell death. These results suggest that the apoptotic effect of ${ alpha}$ -zingiberene on SiHa cells may converge caspase-3 activation through the release of mitochondrial cytochrome c into cytoplasm. It is considered that anti-proliferative effect of ${ alpha}$ -zingiberene is a result of apoptotic effects, and ${ alpha}$ -zingiberene is worth furthermore study to develop it as cancer chemotherapeutics. |
