- HOME
- 홈페이지 이용안내
논문 기본정보
체세포 핵치환 기술을 이용한 hGCSF와 GFP 유전자 동시발현 형질전환 소 배아 생산
논문 개요
| 기관명 | NDSL |
|---|---|
| 저널명 | 韓國受精卵移植學會誌 = Journal of embryo transfer |
| ISSN | 1225-4991, |
| ISBN |
논문저자 및 소속기관 정보
| 저자(한글) | 양정석,조소영,구본철,허영태,이수민,강만종,송혁,고대환,엄상준 |
|---|---|
| 저자(영문) | |
| 소속기관 | |
| 소속기관(영문) | |
| 출판인 | |
| 간행물 번호 | |
| 발행연도 | 2015-01-01 |
| 초록 | The purpose of this study is to develop transgenic cell line expressing targeted human granulocyte colony stimulating factor (hGCSF) and green fluorescence protein (GFP) genes as well as production of Somatic Cell Nuclear Transfer (SCNT) embryos derived from co-expressed transgenic donor cells. Constructed pPiggy-mWAP-hGCSF-EF1-GFP vector was chemically transfected into bovine fetus cells and then, only GFP expressed cells were selected as donor cells for SCNT. Cleavage and blastocyst rates of parthenogenetic, SCNT embryos using non-TG cell and hGCSF-GFP dual expressed SCNT embryos were examined (cleavage rate: $78.0{ pm}2.8$ vs. $73.1{ pm}3.2$ vs. $70.4{ pm}4.3%$ , developmental rate: $27.2{ pm}3.2$ vs. $21.9{ pm}3.1$ vs. $17.0{ pm}2.9%$ ). Result indicated that cleavage and blastocyst rates of TG embryos were significantly lower (P |
| 원문URL | http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=03553784&target=NART&cn=JAKO201507455618037 |
| 첨부파일 |
추가정보
| 과학기술표준분류 | |
|---|---|
| ICT 기술분류 | |
| DDC 분류 | |
| 주제어 (키워드) | SCNT,hGCSF,GFP,transgenic embryo,cow |