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논문 기본정보
뉴트라비딘 검출용 간이 진단키트의 성능향상을 위한 기본 플랫폼 선정
논문 개요
| 기관명 | NDSL |
|---|---|
| 저널명 | 농업과학연구 = CNU Journal of agricultural science |
| ISSN | 1225-2220, |
| ISBN |
논문저자 및 소속기관 정보
| 저자(한글) | 최선미,김기영,엄애선,문지혜,박샛별,이상대,김혁주 |
|---|---|
| 저자(영문) | |
| 소속기관 | |
| 소속기관(영문) | |
| 출판인 | |
| 간행물 번호 | |
| 발행연도 | 2012-01-01 |
| 초록 | This study was performed to optimize the basic platform of a lateral flow immunoassay. Improvement of the limit of detection (LOD) was evaluated according to the width of a nitrocellulose membrane with varying concentrations of analyte. The analyte, neutravidin was detected based on the avidin-biotin interaction. The antibody-Au nanoparticle conjugation was mostly stabled in a PBS buffer of pH 7.3. The optimal widths of a nitrocellulose membrane were 4 and 6 mm considering the sample flow rate and signal strength of the test line on the membrane. The LOD of neutravidin was 0.001 mg/ml in the optimum conditions. |
| 원문URL | http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=03553784&target=NART&cn=JAKO201213660552706 |
| 첨부파일 |
추가정보
| 과학기술표준분류 | |
|---|---|
| ICT 기술분류 | |
| DDC 분류 | |
| 주제어 (키워드) | Rapid detection kit,Nitrocellulose membrane,Avidin-biotin interaction |