| 초록 |
An oversupply of nutrients (crude protein and phytase) in broiler diets reduces the feed efficiency and increases nitrogen and phosphorus excretion in manure. Therefore, the environmental load is increased. A study was conducted to reduce the environmental load due to the excretion of nitrogen and phosphorus by increasing the nutrient utilization rate with the content of feed ingredients suitable for the growth stage of broilers. In this study, the gene expression patterns and response pathways of significantly differentially expressed genes (DEGs) according to the addition of crude protein and phytase in the feed for 36 broiler chickens at one week of age were analyzed by transcriptome (RNA-seq, edge-R) and a functional study. The expression of 131 DEGs related to the energy metabolism decreased in the cecum of one-week-old broilers as the amount of phytase (from 1000ppm to 500ppm) added to the feed was increased. These DEGs help generate ATP energy through 1) protein autophosphorylation and 2) oxidative phosphorylation by kinase, which is the reverse reaction of phytase. In addition, gene expression was activated in the glucose synthesis pathway through 3) the phosphorylation of monosaccharides (fructose or mannose) by kinase. These results will provide information on how the function of energy metabolism is regulated and how the growth is affected by the use of nutrients according to the level of phytase addition in the feed of one-week-old broilers. |
