| 저자(한글) |
LU, Xuedong,ZHOU, Yiping,YANG, Laizhi,LIU, Jian,WEI, Jiehong,HUANG, Lie,HAN, Jian |
| 초록 |
OBJECTIVE To find a method for rapid detection of multiple respiratory pathogens in clinical specimens. METHODS The specific tiny microspheres, multiplex PCR technology and Luminex xMAP (flexible Multi-Analyte Profiling) were used in combination for rapid detection of 14 respiratory pathogens(18 typing) by DNA or RNA. RESULTS The specificity of the diagnostic system had been validated by 15 species of pathogens (19 typing) from ATCC. There was not cross-reaction in each other. In 138 samples collected from clinical respiratory tract infections, bacteria were detected in 26 (37. 68%) of 69 pathogens from 112 bronchoalveolar lavage, including 13 (18. 84%) of Mycobacterium tuberculosis and 44.93% of viral pathogens, and 17.39% of Mycoplasma pneumoniae and Chlamydia pneumoniae. Influenza A virus detected in bronehoalveolar lavage and 26 samples of nasopharyngeal swabs were 21 of 112 (18. 75%) and 7 of 26 (26. 92%), respectively by Luminex xMAP. CONCLUSIONS Luminex xMAP Multi-Analyte Profiling is highly sensitive and accurate, high throughput and increases assay speed for detecting multiple respiratory pathogens in clinical specimens. It is a useful tool for epidemiology yet. |
