| 초록 |
OBJECTIVE To detect the genes of plasmid-mediated AmpC and ESBL β-lactamases and class I integron in clinical isolate of Escherichia coll. METHODS The bacterial susceptibility to antimicrobial agents for strain E50 was determined by agar dilution method. The genes of AmpC, ESBLs, and int Ⅰ 1 were analyzed using PCR and verified DNA sequencing. Conjugation experiment was used to study the transfer of drug resistance. RESULTS The strain was only susceptible to imipenem. CTX-M-15, SHV-1, TEM-1, DHA-1 and int Ⅰ 1 genes were positive. CONCLUSIONS It has complicated mechanism of drug resistance. Some of clinical strains producing plasmid-mediated AmpC β-lactamase are accompanied by extensively producing ESBLs. Carbapenems are the best choice for treatment infection caused by strains producing ESACs. More attention should be paid to integron which plays an important role in multi-resistance. |
