| 저자(한글) |
Yu, J.,Yang, H.,Li, K.,Lei, J.,Zhou, L.,Huang, C. |
| 초록 |
Objectives: To fabricate a nanohydroxyapatite/mesoporous silica nanoparticle (nHAp male;SN) biocomposite and investigate its effectiveness on dentinal tubule occlusion, acid-resistant stability, and microtensile bond strength (MTBS). Methods: The nHAp male;SN biocomposite was synthesized and characterized by X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, N 2 adsorption-desorption isotherms, field-emission scanning electron microscopy (FESEM), and high-resolution transmission electron microscopy (HRTEM). Thirty-two simulated sensitive dentin discs were prepared and randomly divided into four groups according to the following treatments (n=8 each): Group 1, no treatment; Group 2, NovaMin, 15 sx2; Group 3, MSN, 15 sx2; Group 4, nHAp male;SN, 15 sx2. Then, four discs per group were post-treated with 6wt.% citric acid challenge to test their acid-resistant stability. The effects on dentinal tubule occlusion were observed by FESEM. A self-etch adhesive (G-Bond) was applied to evaluate the MTBS. The cytotoxicity was detected using the Cell Counting Kit-8 (CCK-8) assay. Results: Results revealed that the nHAp male;SN biocomposite was successfully fabricated. nHAp male;SN could effectively occlude the dentinal tubules, and the intratubular crystals were tightly associated with the tubular wall. After citric acid attack, nHAp male;SN exhibited the highest acid-resistant stability among the four groups. Moreover, no significant difference in MTBS was noted among the four groups (P gt;0.05). CCK-8 assay identified that nHAp male;SN induced no more than 20% cell death even at the highest concentration of 640 mu;g/mL. Conclusions: The application of the nHAp male;SN biocomposite resulted in efficient dentinal tubule occlusion, acid-resistant stability, and did not compromise immediate bond strength between dentin and self-etch adhesive system. Clinical significance: The nHAp male;SN biocomposite indicates enormous potential as a new strategy for relieving dentin hypersensitivity. |
