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논문 기본정보
Mucoid Pseudomonas aeruginosa Containing a Newly Discovered Mutation of mucA Gene-Its Biofilm Characterization
논문 개요
| 기관명 | NDSL |
|---|---|
| 저널명 | 中華醫院感染學雜志 = Chinese journal of nosocomiology |
| ISSN | 1005-4529, |
| ISBN |
논문저자 및 소속기관 정보
| 저자(한글) | Chen, Anqun,Tian, Deying,Song, Shihui,Wu, Huiling,Ni, Ming,Kalai, Mathee |
|---|---|
| 저자(영문) | |
| 소속기관 | |
| 소속기관(영문) | |
| 출판인 | |
| 간행물 번호 | |
| 발행연도 | 2008-01-01 |
| 초록 | OBJECTIVE To learn the effect of the newly discovered mution in mucA on the biofilm of the Pseudo- monas aeruginosa by observing the biofilm of the mucoid P. aeruginosa PAE17 which contains a newly discovered mutation of mucA gene under different growth conditions. METHODS Using PIA agar to observe the Alg~ phenotype, applying tryptophan reaction to quantitate the glycoealyx. Employing Congo red stain method and stand culture to observe biofilms in solid and liquid conditions respectively. Applying modified plate method to establish biofilm model in vitro, using confocal laser scanning microscope to further observe the morphology of the biofilm with different ages by electroporating the GFP-expressing plasmid (pGFPuv) into the above three strains. RESULTS PAE17 and PDO300 displayed the obvious mucoid phenotype and both had much higher production of glycocalyx (101. 6 μg/ml and 118. 3 μg/ml) than PAEO_1 (56. 3μg/ml), however PAE17 displayed totally different morphology of biofilm from PDO300 in different conditions, inversely which was similar to nomucoid strain PAEO_1. CONCLUSIONS The newly discovered mutation of mucA might have other pathways other than alginate to regulate the formation of biofilm. |
| 원문URL | http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=03553784&target=NART&cn=NART51531215 |
| 첨부파일 |
추가정보
| 과학기술표준분류 | |
|---|---|
| ICT 기술분류 | |
| DDC 분류 | |
| 주제어 (키워드) | Mutation of mucA gene,Pseudomonasaeruginosa,Biofilm,Confocal laser scanning microscope |