| 저자(한글) |
Kumar, A.,Kumar, A.,Gupta, R.K.,Paitandi, R.P.,Singh, K.B.,Trigun, S.K.,Hundal, M.S.,Pandey, D.S. |
| 초록 |
Synthesis, characterization, DNA and protein binding as well as anticancer activity of the organometallic complexes [( eta; 6 -C 6 H 6 )RuCl(APBI)]Cl (1), [( eta; 6 -p-MeC 6 H 4 Pr i )RuCl(APBI)]Cl (2), [( eta; 6 -C 6 Me 6 )RuCl(APBI)]Cl (3), [( eta; 5 -C 5 Me 5 )RhCl(APBI)]Cl.H 2 O (4) and [( eta; 5 -C 5 Me 5 )IrCl(APBI)]Cl.H 2 O (5) containing 2-aminophenyl benzimidazole (APBI) have been described. The complexes 1-5 exhibited strong DNA, protein binding and anticancer activity against cervical cancer (SiHa) cell line. Their binding with calf thymus DNA (CT-DNA) and bovine serum albumin (BSA) have been examined by absorption and emission spectral studies. Strong interactions between complexes and CT-DNA have been affirmed by absorption spectral and EthBr displacement studies, while interaction with BSA via static quenching explored by fluorescence titration, synchronous and 3D fluorescence spectroscopy. The interactions between 1-5 and DNA has also been scrutinized by 1 H NMR spectral studies using guanosine as a model for DNA. These results have been supported by DFT calculations and molecular docking studies. Cytotoxicity, apoptosis and in vitro anticancer activity of 1-5 toward SiHa cell line have been investigated by MTT assay and acridine (AO)/ethidium bromide (EthBr) fluorescence staining. Overall results revealed that DNA and protein binding, as well as anticancer activity of 1-5 follows the order as 5 gt; 3 gt; 2 gt; 1 gt; 4. |
