Kluyveromyces fragilis의 Alkaline Phosphatase 유전자의 E. coli 및 S. cerevisiae 에서의 발현
기관명 | NDSL |
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저널명 | 산업미생물학회지 = Korean journal of applied microbiology and biotechnology |
ISSN | 0257-2389, |
ISBN |
저자(한글) | Park, Soo-Young,Hwang, Seon-Kap,Lee, Dong-Sun,Kim, Jong-Guk,Nam, Joo-Hyun,Hong, Soon-Duck,경북대학교 자연과학대학 미생물학과,경북대학교 자연과학대학 미생물학과,경북대학교 자연과학대학 미생물학과,경북대학교 자연과학대학 미생물학과,대구공업전문대학 식품공업과,경북대학교 자연과학대학 미생물학과 |
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저자(영문) | |
소속기관 | |
소속기관(영문) | |
출판인 | |
간행물 번호 | |
발행연도 | 1995-01-01 |
초록 | The alkaline phosphatase (K-ALPase) gene of Kluyveromyces fragilis has been cloned (1) and determined its base sequences (2) previously in our laboratory. When the K-ALPase gene was expressed in Escherichia coli and Saccharomyces cerevisiae, it showed a constitutive activity in E. coli, and a derepressed activity in S. cerevisiae in phosphate-limited medium. Northem hybridization experiment was performed to elucidate the transcription level of the K-ALPase gene. Northern experiment showed that transcription level of K-ALPase gene in S. cerevisiae was higher in phosphate depletion, but it was higher in high phosphate medium than in phosphate limited medium in K. fragilis. The transcription initiation site of the K-ALPase gene was determined by primer extension analysis. It matched nucleotide position - 169 in relation to the putative trnslational start site. |
원문URL | http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=03553784&target=NART&cn=JAKO199511920018217 |
첨부파일 |
과학기술표준분류 | |
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ICT 기술분류 | |
DDC 분류 | |
주제어 (키워드) | K. fragilis,alkaline phosphatase,gene expression |