| 저자(한글) |
ZHANG, Hongming,HUANG, Qing,LUO, Yang,YAO, Chunyan,WANG, Guangjie,FU, Weiling |
| 저자(영문) |
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| 소속기관 |
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| 소속기관(영문) |
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| 출판인 |
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| 간행물 번호 |
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| 발행연도 |
2008-01-01 |
| 초록 |
?BJECTIVE To characterize bacteriophage isolated from sewage with indicator of Klebsiella pneumoniae subsp pneumoniae. METHODS Bacteriophage was isolated from sewage by double-layer agar plate method, identified lytic or lysogenic capacity by induced methods of ultraviolet ray and mitomycin C, performed one-step growth experiments, decided the optimal multiplicity of infection, and its structure was observed with electron microscope. RESULTS The study found a strain of bacteriophage against the K. pneumoniae subsp pneumoniae. The phage had a head of 180 nm in diameter and a tail of 210 nm in length. The plaque was transparent and 4-7 mm in diameter, the optimal multiplicity of infection was 4, latent period was 35 min and burst period was 40 min, the average burst size was about 94 PFU/cell. CONCLUSIONS The isolated bacteriophage belongs to Stylovinidae, and it is a lytic bacteriophage with narrow host spectrum. Moreover, it is only sensitive to a part of K. pneumoniae subsp pneumoniae and a few of Escherichia coli strains. |
| 원문URL |
http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=03553784&target=NART&cn=NART51531074 |
| 첨부파일 |
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