| 저자(한글) |
Yang, Mingjuan,Ke, Yuehua,Wang, Xuesong,Ren, Hang,Liu, Wei,Lu, Huijun,Zhang, Wenyi,Liu, Shiwei,Chang, Guohui,Tian, Shuguang,Wang, Lihua,Huang, Liuyu,Liu, Chao,Yang, Ruifu,Chen, Zeliang |
| 초록 |
Confirming Ebola virus disease (EVD), a deadly infectious disease, requires real-time RT-PCR, which takes up to a few hours to yield results. Therefore, a rapid diagnostic assay is imperative for EVD diagnosis. A rapid nucleic acid test based on recombinase polymerase amplification (EBOV-RPA) was developed to specifically detect the 2014 outbreak strains. The EBOV-RPA assay was evaluated by testing samples from suspected EVD patients in parallel with RT-PCR. An EBOV-RPA, which could be completed in 20 thinsp;min, was successfully developed. Of 271 patients who tested positive for Ebola virus by RT-PCR, 264 (sensitivity: 97%, 95% CI: 95.5 ndash;99.3%) were positive by EBOV-RPA; 101 of 104 patients (specificity: 97%, 95% CI: 93.9 ndash;100%) who tested negative by RT-PCR were also negative by EBOV-RPA. The sensitivity values for samples with a Ct value of |
