| 저자(한글) |
Woo, Su-Mi,Lim, Hae-Soon,Jeong, Kyung-Yi,Kim, Seon-Mi,Kim, Won-Jae,Jung, Ji-Yeon |
| 초록 |
The active metabolite of vitamin D such as $1{ alpha}$ ,25-dihydroxyvitamin ( $D_3(1{ alpha},25(OH)_2D_3)$ is a well-known key regulatory factor in bone metabolism. However, little is known about the potential of vitamin D as an odontogenic inducer in human dental pulp cells (HDPCs) in vitro. The purpose of this study was to evaluate the effect of vitamin $D_3$ metabolite, $1{ alpha},25(OH)_2D_3$ , on odontoblastic differentiation in HDPCs. HDPCs extracted from maxillary supernumerary incisors and third molars were directly cultured with $1{ alpha},25(OH)_2D_3$ in the absence of differentiation-inducing factors. Treatment of HDPCs with $1{ alpha},25(OH)_2D_3$ at a concentration of 10 nM or 100 nM significantly upregulated the expression of dentin sialophosphoprotein (DSPP) and dentin matrix protein1 (DMP1), the odontogenesis-related genes. Also, $1{ alpha},25(OH)_2D_3$ enhanced the alkaline phosphatase (ALP) activity and mineralization in HDPCs. In addition, $1{ alpha},25(OH)_2D_3$ induced activation of extracellular signal-regulated kinases (ERKs), whereas the ERK inhibitor U0126 ameliorated the upregulation of DSPP and DMP1 and reduced the mineralization enhanced by $1{ alpha},25(OH)_2D_3$ . These results demonstrated that $1{ alpha},25(OH)_2D_3$ promoted odontoblastic differentiation of HDPCs via modulating ERK activation. |
