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논문 기본정보
Enhanced Healing of Rat Calvarial Bone Defects with Hypoxic Conditioned Medium from Mesenchymal Stem Cells through Increased Endogenous Stem Cell Migration via Regulation of ICAM-1 Targeted-microRNA-221
논문 개요
| 기관명 | NDSL |
|---|---|
| 저널명 | Molecules and cells |
| ISSN | 1016-8478,0219-1032 |
| ISBN |
논문저자 및 소속기관 정보
| 저자(한글) | Chang, Woochul,Kim, Ran,Park, Sang In,Jung, Yu Jin,Ham, Onju,Lee, Jihyun,Kim, Ji Hyeong,Oh, Sekyung,Lee, Min Young,Kim, Jongmin,Park, Moon-Seo,Chung, Yong-An,Hwang, Ki-Chul,Maeng, Lee-So |
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| 저자(영문) | |
| 소속기관 | |
| 소속기관(영문) | |
| 출판인 | |
| 간행물 번호 | |
| 발행연도 | 2015-01-01 |
| 초록 | The use of conditioned medium from mesenchymal stem cells may be a feasible approach for regeneration of bone defects through secretion of various components of mesenchymal stem cells such as cytokines, chemokines, and growth factors. Mesenchymal stem cells secrete and accumulate multiple factors in conditioned medium under specific physiological conditions. In this study, we investigated whether the conditioned medium collected under hypoxic condition could effectively influence bone regeneration through enhanced migration and adhesion of endogenous mesenchymal stem cells. Cell migration and adhesion abilities were increased through overexpression of intercellular adhesion molecule-1 in hypoxic conditioned medium treated group. Intercellular adhesion molecule-1 was upregulated by microRNA-221 in mesenchymal stem cells because microRNAs are key regulators of various biological functions via gene expression. To investigate the effects in vivo, evaluation of bone regeneration by computed tomography and histological assays revealed that osteogenesis was enhanced in the hypoxic conditioned medium group relative to the other groups. These results suggest that behavioral changes of endogenous mesenchymal stem cells through microRNA-221 targeted-intercellular adhesion molecule-1 expression under hypoxic conditions may be a potential treatment for patients with bone defects. |
| 원문URL | http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=03553784&target=NART&cn=JAKO201523964822445 |
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추가정보
| 과학기술표준분류 | |
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| ICT 기술분류 | |
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| 주제어 (키워드) | bone regeneration,calvarial defect model,hypoxic conditioned medium,intercellular adhesion molecule-1,mesenchymal stem cells,microRNA-221 |