| 초록 |
Insulin-like growth factor-I(IGF-I) plays an important role in the regulation of mammalian and poultry growth. IGF-I has many actions in different tissues, which include metabolic, mitogenic, and differentiative actions. IGF-I induces insulin-like effects - such as increased cell glucose uptake and glycogen sysnthesis, however several physiological actions of IGF-I may not have been identified yet. In order to investigate the effect on growth in broiler chicken treated with exogenous insulin-like growth factor-I, 30 chickens were injected $50{ mu}g$ reconbinant human IGF- I (rhIGF- I ) per kg body weight as experimental group and 30 ckickens saline subcutanously as control, 3 times according to ages from 2 to 35 days. We established radioimmunoassay method by which we can measure chicken IGF- I (cIGF- I ) as in rhIGF- I assay. The results obtained were as follows; 1) The dilution curve showed in parallelism between rhIGF- I and cIGF- I in the Sep-pak $C_{18}$ cartridge plasma extracts. 2) The body weight of broiler chicken were significantly increased at 31 days( $1,176.50{ pm}99.79g$ ) and 35 days( $1,252.84{ pm}125.21g$ ) of age in treatment groups, compared with control group( $1,011.88{ pm}40.22g, ;1,111.32{ pm}153.67g$ ). The liver and kidney weights on 35 days $(35.24{ pm}5.18g, ;11.05{ pm}1.47g)$ were significantly higher in rhIGF- I treated group than control group( $30.95{ pm}4.04g, ;10.01{ pm}1.60g$ ) 3) The plasma concentration of IGF- l and total protein in rhIGF- I treated group were $58.17{ pm}1.69ng/ml$ , $3.75{ pm}0.62g/dl$ respectively compared with control group $45.70{ pm}1.64ng/ml$ , $2.32{ pm}0.53g/dl$ . The results suggest that exogenous rhIGF- I increased total body weight, liver and kidney weights in broiler chicken, and it may increase IGF- I and total protein concentration in serum. |
