| 초록 |
Objectives : The purpose of this study was to investigate the antioxidant activity of water extract of Woogyuyeum (WGY) in Leydig cells. Methods : We investigated the cytoprotective effect of WGY in cultured mouse Leydig cells by MTT assay. Leydig cells treated with WGY were incubated in the presence or absence of 50 #x3BC;M hydrogen peroxide at 37 #x2103; for 24 h. The protective effects of WGY against hydrogen peroxide-induced oxidative stress, lipid peroxide (LPO), superoxide dismutase (SOD), and catalase activity assays were performed in Leydig cells. Results : As a result, WGY showed no significant cytotoxicity in Leygdig cells. WGY showed cell viability as 103.65% in 5 #x3BC;g/ml concentrations. The cytotoxicity induced by hydrogen peroxide in Leygdig cells, the antioxidant effects of WGY was increased in 1, 5, 50, 100 ug/ml concentraions. 100 #x3BC;g/ml concentration of WGY showed maximum antioxidant effects. Treatment of cells with 100 #x3BC;g/ml WGY significantly reduced the MDA concentration to 0.23 nmoles/mg protein. SOD activity was increased at 1, 100 #x3BC;g/ml concentration of WGY and catalase activity was significantly increased at 50, 100 #x3BC;g/ml concentrations of WGY, respectively. Conclusions : In conclusion, WGY has antioxidant activities against hydrogen peroxide-induced oxidative stress in Leydig cells. |
